重度创伤失血性休克,动物造模 z-bio 2021-11-03 579

　　Objective: To establish an improved rat model of severe traumatic shock (STHS), and to explore the protective effect of anesthesia on the STHS rat model.

　　Method: 40 adult healthy male SD rats were randomly divided into 4 groups. The normal group was anesthetized with bilateral femoral arteries, and the left femoral vein was intubated for anesthesia. After the rats regained or maintained anesthesia, they were divided into wake-up control group. Severe use of open abdominal wounds and combined use (CC, n = 10), anesthesia control group (AC, n = 10), traumatic shock group, the method of regular and quantitative control of blood loss during anesthesia or wakefulness established traumatic hemorrhagic shock The mouse model is the traumatic hemorrhagic shock group during awakening (CTHS, n = 10) and the traumatic hemorrhagic shock group during anesthesia (ATHS, n = 10). During the experiment, observe the overall physiological condition of each group of rats, and continuously monitor the hemodynamic indicators of experimental rats: mean arterial pressure (MAP), heart rate (HR), left ventricular pressure. Maximum increase/decrease rate (±dp/dtmax)), arterial blood gas index: pH, oxygen partial pressure (PaO2), carbon dioxide partial pressure (PaCO2), hemoglobin measurement (ctHb), lactic acid (Lac), blood glucose (Glu)) , Bicarbonate concentration (cHCO3-), alkaline residue (BE); at the end of the experiment, damage to the rat heart, liver, lungs and kidney organs was observed under the HE staining microscope.

　　Results: In the CTHS and ATHS groups, there were significant differences in the changes of HR and ±dp/dtmax over time (P\u003c0.01). The HR and ±dp/dtmax of the ATHS group showed a downward trend, while the CTHS group showed a downward trend. High or steady state (T2, T3, T4, T5, T6) means first decline (T1) and then rise; after the ATHS group is impacted, the MAP shows a gradual upward trend, and finally is 25%-40. Level%. On the other hand, after the compensatory growth period, the CTHS group declined rapidly. PH, PaCO2, ctHb, cHCO3- and BE in CTHS and ATHS groups showed a gradual decrease. Except for PH and ctHb, the decrease in CTHS group was significantly greater than that in ATHS group (P\u003c0.01); Glu, Lac and PaO2 showed a gradual upward trend, while the increase in CTHS group was significantly higher than that of ATHS group (P\u003c0.01). Compared with the CTHS group, the ATHS group significantly reduced liver, heart, lung and kidney damage.

　　Conclusion: Anesthesia can help protect rats from severe hemorrhagic shock, improve hemodynamics, reduce the accumulation of respiration and metabolites, and reduce the degree of damage to various organs.