非酒精性脂肪肝病模型,动物造模 z-bio 2021-12-21 791

　　Objective: To establish a new type of hyperlipidemia (WSHc) rat non-alcoholic fatty liver disease (NAFLD) model, observe the changes in blood biochemical indicators and pathological characteristics, and apply and study a sick animal model, which explores Has gained value and provided it with a new option to become NAFLD.

　　Method: 27-8 weeks old WSHc rats were randomly divided into two groups, fed with a normal diet and a high-fat diet. Another 20 Wistar rats of the same age were harvested and treated in the same way as the strain control. After 12 weeks of continuous feeding, the serum TC, TG, ALT and AST levels were detected, and elastic ultrasound imaging was performed. After the animals were sacrificed, the liver was harvested for HE, Oil Red O, Masson and immunofluorescence staining, and the liver morphology, lipid deposition, inflammation and fibrosis of each group were observed. The liver of WSHc rats after high fat. The diet was analyzed. The characteristics of the lesion. Compared with Wistar rats on a high-fat diet, WSHc rats are induced by high fat, similar to clinical hyperlipidemia patients, and have a significant increase in serum total cholesterol; abnormal liver lipid deposits and bullous lipid drops , Inflammatory cells infiltrate the surrounding. Lipid droplets in the environment form inflammatory lesions and have more macrophages; scattered fibrous lesions can be seen. However, these pathological changes were not observed in the liver tissue of Wistar rats induced by high fat.

　　Conclusion: Compared with Wistar rats, the blood lipids and liver enzyme levels of WSHc rats after hyperlipid induction are close to clinical levels, lipid deposition and inflammation in liver tissues are more serious, and liver fibrosis is scattered. The new ideal NAFLD animal model.