局灶性脑缺血模型,动物造模 z-bio 2021-11-06 498

　　Objective: To observe the feasibility of electrocoagulation on the middle cerebral artery in mice, occlude the middle cerebral artery, and provide the possibility to establish a mouse cerebral ischemia model.

　　Method: Use electrocoagulation to directly block the middle cerebral artery (MCA), and create a Balb/c mouse cerebral ischemia model in adult males (model group, n = 20). At the same time, create the same batch of Balb/c. The mice had the same craniotomy, but without electrocoagulation and middle cerebral artery occlusion, and were treated as a sham operation group (sham operation group, n = 20). The neurological deficit score (mNSS) was used to evaluate the nerve damage in the model and sham operation groups at 24 and 72 hours after surgery.

　　Result: Twenty-four hours after the operation, brain tissue sections were obtained from the injured area of Balb/c mice in the model group and stained with hematoxylin-eosin. Microscopic examination showed ischemic interstitial edema of brain tissue. When there is a cavity, the structure of the local brain tissue becomes loose, the color becomes lighter, the number of nerve cells is significantly reduced, the body of the nerve cells shrinks, and the outline of some cells becomes unclear. Cell degeneration and necrosis, nuclear sepsis and loss of nucleoli can be seen to varying degrees. After 24 hours, the neurological deficit score of the model group was significantly lower than that of the sham operation group (P\u003c0.05), and this difference can be maintained until 72 hours after surgery.

　　Conclusion: Electrocoagulation can successfully create a mouse model of local cerebral ischemia in Balb/c mice.