坏死性小肠结肠炎模型,动物造模 z-bio 2021-12-07 959

　　Objective: To improve and compare the methods for establishing necrotizing enterocolitis (NEC) animal models commonly used at home and abroad, and to clarify simple and effective NEC modeling methods.

　　Method: Use newborn SD rats within the first 2 hours of life and divide them into 5 groups randomly. There are 10 animals in the control group and 20 animals in each group in the experimental group. Breastfeeding group A and surrogate rats in the same cage without intervention; group B artificial feeding + cold hypoxia stimulation + (lipopolysaccharide) LPS gavage (5 mg/kg body weight); group C artificial feeding + hypoxia cold Stimulation + LPS forced feeding (10 mg/kg); Group D artificial feeding + hypoxic cold stimulation + LPS intraperitoneal injection (2 mg/kg); E group artificial feeding + hypoxia cold stimulation + LPS intraperitoneal injection (5 mg /kg). Observe the daily activities and weight changes of newborn rats. After the experiment, in order to assess the degree of pathological damage to the small intestine, small intestine tissue was collected for hematoxylin-eosin staining, and the level of tumor necrosis factor-α (TNF-α) in the small intestine tissue was detected.

　　Result: The newborn rats in the experimental group showed varying degrees of hypofunction, abdominal distension, diarrhea, black stool and weight loss. The pathological score showed that the pathological damage score of the experimental group was significantly higher than that of the control group (P\u003c0.05). The incidence of NEC in the LPS intraperitoneal injection group (D, E) was higher than that in the oral administration group (B, C) (P\u003c0.05), but there was no NEC related to the LPS high-dose (5 mg/kg) intraperitoneal injection group The mortality rate was significantly higher than other groups (P\u003c0.05).

　　Conclusion: The method of combining artificial nutrition and hypoxic cold stimulation with low-dose LPS (2 mg/kg) intraperitoneal injection to establish NEC is easier to operate and stable.