压力超负荷心衰小鼠模型,动物造模 z-bio 2021-10-12 509

　　Objective: To establish a mouse heart failure model, and to use ultrasound images and pathological techniques to comprehensively evaluate the characteristic changes in cardiac function and structure during the onset of the disease.

　　Method: The aortic arch stenosis (TAC) surgical technique is used to establish a mouse model of heart failure caused by pressure overload. The model is performed 0 weeks before the appearance of the model and 2, 4, blood flow Doppler of the aortic valve stenosis. collect. Estimate the vascular pressure and collect B-mode and M-mode ultrasound images to evaluate the left ventricular systolic function of the heart structure, and combine the mitral valve annulus to collect the mitral annulus blood flow Doppler. Tissue Doppler evaluates extended functions and collects heart tissue. Histopathological observation.

　　Results: Ultrasound diagnosis showed a significant increase in aortic blood pressure at 2, 4, and 8 weeks after surgery. The main symptoms in the two weeks after surgery are a compensatory increase in the left ventricular wall, a decrease in inner diameter and an increase in compensatory function of systolic function, but impaired diastolic function and myocardial fibrosis. Four weeks after the operation is a transitional period. This is mainly manifested as the transition from ventricular wall hypertrophy to ventricular dilation, systolic function begins to decline, diastolic function continues to be impaired, and the degree of myocardial fibrosis is higher; 8 weeks after surgery, the characteristic ventricular space shows dilation, and the systolic and systolic periods are obvious cut back. Contraction and relaxation function. The cardiomyocytes were partially lysed and obviously fibrotic.

　　Conclusion: The structure and function of the left ventricle were evaluated in three stages: compensatory myocardial hypertrophy, the transition of mice to decompensated dilated heart failure after aortic arch contraction, the basic knowledge of the aortic arch contraction model and translational research, passed the above The application can provide a theoretical basis.