盆腔脏器脱垂,动物模型,动物造模 z-bio 2022-08-22 942

　　Objective: To establish a rat animal model that can reflect similar pathological changes of similar ligaments in patients with pelvic organ prolapse, and to explore the effects of increased birth injury intensity and menopausal simulation on the pathology of rat bone ligaments.

　　Method: 60 SPF-grade SD adult female rats weighing about 300g were selected, 45 of which had no birth history, and were simulated by a blank control group (A), a simulated menopause group (B) and a random number table. It is classified as a birth injury. Methods: In the simulated menopause group (C), the other 15 babies who gave birth 3 times in a row were birth injury + simulated birth injury + simulated menopause group (D). Group A eats and drinks regularly, group B underwent bilateral oophorectomy, group C underwent simulated birth injury surgery, bilateral oophorectomy to simulate menopause, group D underwent bilateral oophorectomy twice after consecutive delivery, and group D underwent Two simulated menopausal oophorectomy. Reproduction is 8-10 weeks after operation. Eight rats were randomly selected from each group, and the changes in the appearance and dormancy of the genitals of the rats in each group were observed. Use immunohistochemical methods to observe and evaluate the type I and type III collagen in the anterior vaginal wall. (COL1A1, COL3A1), transforming growth factor β-3 (TGFβ-3) integrated optical density changes. RT-PCR was used to determine the expression levels of COL1A1, COL3A1 and TGFβ-3mRNA in the ligament tissue.

　　Result: After modeling, the diameter of the vaginal fissure of the rats in the D group was larger than the diameter of the control group by more than 2mm. Rats in each group had no obvious prolapsed phenotype, and group D showed mild perineal abnormalities. The expression of wall COL1A1 was significantly lower than that of group A (P\u003c0.05). Compared with group A, the relative expression of TGFβ-3 in group C and D was significantly higher than that in group A (P\u003c0.05). The expression of COLIA1 and COL3A1 in the ligaments of D rats was significantly lower than that of group A (P\u003c0.05). Compared with group A, the relative expression of TGFβ-3 in group C and D was significantly higher than that in group A (P\u003c0.05). ).

　　Conclusion: The genital prolapse phenotype of the rat model after modeling (for example, the study of phenotypic changes as the research content) is not important, so it is not suitable to create this model. This model can be used as an animal to study pathophysiology. This is a modification of the POP s ligament model, but it cannot be determined whether the mechanical properties of human and rat ligaments are similar. The best animal model should be selected according to the advantages and disadvantages of each different animal and actual research. The problem to be solved.