肝外型胆汁淤积模型,动物造模 z-bio 2021-05-14 535

　　Objective: To evaluate the advantages and disadvantages of three surgical models of extrahepatic cholestasis in rats through serology, diagnostic imaging and pathology.

　　Method: 15 female SD rats were randomly divided into conventional group, modified group and electrosurgery group through common bile duct ligation (n = 5) and common hepatic duct suture (n = 5). , And bile duct ligation (n = 5). Common bile duct radiofrequency electrosurgery and electrocoagulation (n = 5) were used to simulate cholestasis surgery. Before and after the operation, CT scan and weight measurement were performed to compare the liver. Density and diameter of bile duct. After 7 days, 3 groups of rats were bled for measurement. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin (TBIL), direct bilirubin (DBIL), after blood collection, rats died of anesthesia and overdose, accounting for 4.2% of liver tissue To 5.2%. Paraffin sections were fixed with Ermarin solution and stained with HE, and pathological changes were observed.

　　Result: The three groups of rats all developed different degrees of liver cirrhosis and cholestasis 7 days after modeling. The AST, TBIL and DBIL of the traditional group were higher than those of the electrosurgery group (P0.05). Pathology showed that the bile duct hyperplasia was the most serious in the conventional group, and the bile duct wall thickened in the three groups. The CT scan of the routine group showed that the dilated diameter of the extrahepatic bile duct was larger than that of the modified group and the electrosurgery group (P0.05). CT value. The proportion of liver in the traditional group was significantly lower than that before surgery (P0.05).

　　Conclusion: Electrosurgical methods and improved methods are more effective in prolonging the process of cholestasis in rats, better simulating some human extrahepatic cholestasis and cholestasis, providing the possibility for dynamic observation of the pathological process.