门脉高压动物模型,动物造模 z-bio 2021-10-11 821

　　Objective: To investigate whether ligation of the left portal vein can form stable portal hypertension, and whether nitric oxide plays a corresponding role.

　　Method: 36 SPF male 8-week-old SD rats were randomly divided into the left branch ligation group (with needles with diameters of 0.6, 0.7 and 0.8 mm, respectively) and the main ligation group (with a diameter of 0.9 mm). Needle ligation is divided into 6 groups, left lateral lobectomy group and sham operation group. The portal pressure of all rats was measured before operation, after operation, 15 days after operation, and 30 days after operation. On the 30th day after the operation, all rats were sacrificed and portal vein blood and left and right liver tissues were collected to measure the content of nitric oxide (NO, nitric oxide).

　　Result: The portal pressure of SD rats in the left branch ligation group was significantly higher than that of the preoperative and control group on the 15th and 30th day after the operation. The difference between the left branch ligation group and the main pipe was 0.6 mm. At 30 days postoperatively, there was no statistical significance in the 0.9 mm trunk group. There was no statistical difference in NO content in left and right liver tissues and blood of 6 groups of SD rats.

　　Conclusion: Ligation of the left portal artery can form stable portal hypertension, and a needle with a diameter of 0.6 mm can achieve better results. O is not involved in anterior portal hypertension.