咳嗽变异型哮喘豚鼠模型,动物造模 z-bio 2021-10-13 854

　　Objective: Try to use ovalbumin in combination with smoking to establish a more similar guinea pig CVA model.

　　Method: Select 24 SPF male and female guinea pigs and randomly divide them into a blank control group, an OVA sensitization group, an OVA sensitization + smoking group, a total of 3 groups, each with 8 guinea pigs, I divided them into several groups . The OVA sensitization group was modeled by the ovalbumin sensitization method, and the OVA sensitization + smoking group was modeled by combining the ovalbumin sensitization and smoking methods. The other methods are the same. Three groups of guinea pigs were tested for lung compliance (Cydn) after metholine challenge, airway resistance (RI), number of coughs and time to first cough after capsaicin challenge, lung histopathology and Ashcroft scale score index Changes to evaluate the model.

　　Results: Compared with the initial blank control group, the Cydn of the OVA sensitization group and the OVA sensitization + smoking group were significantly reduced, the RI was significantly increased, and the number of coughs at 10 minutes was significantly increased. Initially, the coughing time was significantly longer fast. , The proportion of pathologically changed lung tissue is clear, the score on the Ashcroft classification table is significantly increased, and the above performance is consistent with the characteristics of cough and asthma. At the same time, compared with the OVA sensitization group, the OVA sensitization + smoking group performed better in terms of cough symptoms and RI changes, which is more in line with the characteristics of cough and asthma. This may be related to the decrease in OVA. In order to avoid excessive immune response, it should be noted that the concentration of dense smoke is closely related to the increase in cough sensitivity.

　　Conclusion: The combination of OVA sensitization method and smoking can establish a highly similar cough asthma guinea pig model.