阿尔茨海默病模型,动物造模 z-bio 2023-01-17 797

　　OBJECTIVE: To explore the effect of jujube saponins (ZSS) on reducing the anesthesia effect of sevoflurane in 9-month-old APP/PS1/tau3 transgenic Alzheimer's disease model mice.

　　Method: According to the principle of random allocation, 9-month-old male APPSwe/PS1M146V/tauP301L3xTg-AD3xTg-AD mice and their background C57BL/6J mice were divided into 4 groups, each with 12 mice. Group Con (saline), 3 rounds of model control group, ModelCon (saline), 3 rounds of model anesthesia group 1 (saline + sevoflurane), 3 rounds of model administration group 2 (saline + sevoflurane + ZSS). All mice were injected with the same amount of normal saline or 0.1μg/4μL jujuboside from the lateral ventricle for 10 consecutive days. The mice in the control group inhaled oxygen for 2 hours, and the anesthesia and administration groups inhaled sevoflurane for 2 hours. Twenty-four hours after the end of anesthesia, six mice in each group were used in the Morris water maze to test their spatial learning and memory capabilities. In other mice, fresh hippocampal tissue was obtained by hair removal, homogenized, and the b-amyloid peptide (Aβ) aggregation (Aβ, Aβ1-40, Aβ1-42) was detected by Western blotting. At the same time, Analyze the detected tau protein expression and changes in the phosphorylation level of its sites (serine 396, serine 262, threonine 231), and detect mitochondrial apoptosis signaling pathway genes including B lymphocyte tumor 2 by real-time PCR technology . In the expression of genes (BcL-2), BcL-2 is related to protein X (Bax) and caspase-3.

　　Result: The Morris water maze experiment showed that sevoflurane does not affect the learning and memory ability of mice, but it has potential neurotoxicity to mice. Compared with three-transformed model mice, jujube saponins can reduce Aβ aggregation and tau protein phosphate. In the 3-round model anesthesia group, in the 3-round model administration group, the expression of BcL-2 decreased, while the expression of Bax and Caspase-3 increased.

　　Conclusion: Jujubesaponin can reduce the anesthetic effect of sevoflurane, activate mitochondrial-related apoptosis signaling pathways, reduce Aβ aggregation, reduce tau protein phosphorylation, and inhibit apoptotic neurons in the hippocampus of mice.