动物模型,DIC z-bo 2020-08-06 570

　　(1) Reproduction method A male New Zealand rabbit weighing about 2 kg was injected with 250 μg of pathogenic E. coli endotoxin through the ear vein. After 24 hours, the same dose was injected from the ear vein. Observe and record the performance after each injection. 4 hours after the second injection of endotoxin, the rabbits were killed by injecting air from the ear vein, and immediately autopsied for visual observation. Take the kidney tissue for quick frozen section, HE staining, take the kidney, lung, liver tissue for paraffin-embedded sections, respectively, for HE staining, Phosphot-ungstic Acid-Hematoxylin (PTAH) staining and Gram staining, Observe under an optical microscope.

　　(2) Model characteristics This model can induce DIC pathological changes and obvious pathological changes similar to humans in rabbits. The method is simple, the results are reliable, and it is easy to observe. It can be used for general teaching and scientific research.

　　(3) Comparative medicine After the first injection of endotoxin in this model, the function of the model animal’s mononuclear macrophage system is impaired, and the body’s ability to remove infectious toxins, activated coagulation factors, fibrinogen degradation products, etc., is reduced, making the second The injected endotoxin plays a better role in inducing DIC, which is one of the advantages of this experimental method. The later stage of the development of DIC lesions is the secondary hyperfunction of the fibrinolytic system, and most of the microblood test dissolves and disappears. We sacrificed the animals 4 hours after the second injection of endotoxin. The detection rate of microthrombus was high, and it was not seen. Obvious hemolysis and bleeding. This is the second method that this experimental method is superior to other methods. The mechanism of the formation of DIC caused by endotoxin is more complicated, and it is a comprehensive result of many factors, which induces the pathological changes and obvious pathological changes of DIC in rabbits similar to the human body.