兔心肌梗死模型,动物造模 z-bio 2021-05-14 494

　　Objective: To explore the application of cardiac catheterization technique in coronary artery occlusion to establish a rabbit myocardial infarction (MI) model.

　　Method: After general anesthesia, 25 rabbits were separated from the carotid artery, the carotid artery was punctured, and the 5F vascular sheath was inserted and fixed. The guide catheter is delivered from the vascular sheath to the bottom of the sinus at the bottom of the aorta, and the microcatheter is delivered to the center or distal end of the left anterior descending branch using a micro-guide wire and gelfoam particle embolizer or spring. The coil was injected into the left anterior descending artery along the microcatheter, and the left anterior descending artery was occluded to create an MI model. One month later, the heart was removed and a pathological examination was performed.

　　Result: 25 rabbits, 19 rabbits in total, were successfully delivered to the left anterior descending artery of the micro-guide wire and micro-catheter. Among the 10 rabbits in the Spring Coil group, MI successfully produced 7 rabbits and 2 died in captivity. Among the 9 rabbits in the gelatin sponge particle embolization treatment group, 7 successfully developed MI, and 1 rabbit died during feeding. There was no significant difference in the success rate of rabbit myocardial infarction and the area of rabbit myocardial infarction between the two groups.

　　Conclusion: Myocardial infarction model can be established by cardiac catheterization, and conforms to the pathological characteristics of clinical myocardial infarction, such as high success rate, stable characteristics of myocardial infarction and avoidance of thoracotomy injury.