兔窦房结损伤,动物造模 z-bio 2022-01-12 594

　　Objective: To establish a rabbit sinus node injury model induced by formaldehyde.

　　Method: Use 40% formaldehyde solution to damage rabbit sinus node, detect heart rate, sinus conduction time, sinus node recovery time, modify sinus node recovery time and other indicators, compare various damage methods, damage degree, damage duration, And more. , And use positive drugs to validate the model.

　　Result: (1) Damage method: The wet compression method can control the degree of damage over time and area. The instillation method is less traumatic, and the formaldehyde content can be used to control the degree of damage, but it is more likely to damage the tissues around the sinus node; (2) The degree and duration of damage: the heart rate is reduced if the percentage is less than 30% of the basal heart rate, then the model is maintained The time is less than 7 days. When the basal heart rate drops from 30% to 60%, the maintenance time of the model can reach 70 days. When the heart rate drops more than 60% of the basal heart rate, the mortality rate of the model animal is relatively high. (3) Active drug verification: Both atropine and Xinbao pills will increase the heart rate of the sinus node injury model and improve the function of the sinus node.

　　Conclusion: The rabbit sinus node injury model established by the formaldehyde wet pressure method is more reliable than the infusion method. The degree of damage should be controlled by reducing the heart rate between 30% and 60% of the basal heart rate. speed. The model was kept for a long time, and the animal was dead.