兔脑死亡模型,动物造模 z-bio 2021-05-17 771

　　Objective: To explore a method to simulate rabbit brain death by improving intracranial pressure and brain death.

　　Methods: 15 New Zealand white rabbits were randomly divided into two groups: sham operation group (group B, n = 6), only intracranial intubation, anesthesia; brain death group (group A, n = 9)), A used modified Intracranial compression creates a brain death model. Record the changes in mean arterial pressure (MAP) and heart rate (HR). Ventilators and vasoactive drugs are used to maintain vital signs at specific and controllable levels.

　　Result: 8 of the 9 rabbits in group A were successfully modeled. With the increase of intracranial pressure during the process of intracranial pressure, MAP and HR gradually increase in a wave shape. The peak values of MAP under anesthesia and brain death were (80.63±8.45) mmHg and (111.63±7.71) mmHg, respectively, which were statistically significant (P\u003c0.05). At the time of brain death and 2 hours after brain death, the HR under anesthesia was (153.25±14.35) times/minute, (262.38±16.60) times/minute and (218.50±10.27) times/minute, respectively. Statistical difference (P\u003c0.05).

　　Conclusion: Compared with traditional surgical methods, the improved method can establish a stable and reliable rabbit brain death model. Timely and effective breathing and circulatory support can help maintain brain death for a long time.