动物模型,急性DIC模型 z-bo 2020-08-06 604

　　(1) Reproduction method male rats weighing about 200g, after conventional anesthesia, inject 10% polymer dextran (HMWD, molecular weight 400,000) 1.5ml/100g body weight from the tail vein, 15-20 minutes later, observe the microcirculation under a microscope During granular flow, another intravenous injection of 5% calcium gluconate with 0.07～0.1ml/100g body weight will form diffuse intravascular coagulation. In the process of modeling, it should be noted that the polymer dextran should be injected quickly in a short time, because the modeling reagent polymer dextran is easily used by the body, and the slow injection speed will make the modeling failure.

　　(2) Model features The early acute DIC model in rats induced by polymer dextran + calcium gluconate is the increase in blood viscosity through polymer dextran, which leads to microcirculation disorders, and makes blood cells and platelets easy Aggregation, and then induced by calcium gluconate, causes extensive microthrombosis in the microcirculation system. The model has a high success rate, good repeatability, simple and easy to implement, and the modeling reagents are cheap and easy to obtain.

　　(3) Comparative Medicine Clinical DIC is a complex disease with multiple etiologies and biochemical abnormalities. The pathological basis is acute and extensive activation of the coagulation and fibrinolysis system, which disrupts the fine balance between thrombosis and clot dissolution. The DIC model caused by polymer dextran + calcium gluconate is susceptible to the bolus injection rate of polymer dextran, and the platelet count and hemoglobin content of the modeled animals decrease. A large number of abnormal red blood cells appeared in blood smears, of which schistosome cells exceeded 1%. By the second day of treatment, in addition to schistosome cells, more target red blood cells were seen. This result proves that experimental animals have developed microangiopathic hemolytic anemia (MHA), which is similar to the appearance of platelets, hemoglobin and schistosome cells after clinical DIC.