绒毛膜癌移植瘤病理模型,动物造模 z-bio 2021-10-08 412

　　Objective: To establish a stable and effective choriocarcinoma xenograft model, and observe the course characteristics and biological behavior of the model.

　　Methods: 3~5 weeks old SCID beige female mice were randomly divided into experimental group and control group; 12 mice in the experimental group were divided into A (subcutaneous transplantation tumor model)/B (pulmonary metastasis model) two groups, respectively subcutaneously Injection or tail vein injection of 5×106 JAR cells per mouse, using morphology, radioimmunoassay to determine β-HCG, small animal in vivo imaging system (IVIS) and histological HE staining methods to identify JAR cells subcutaneously transplanted tumors and lung metastases Formation and so on.

　　Results: 28 days after inoculation in the experimental group, mice in group A formed hard tumor nodules under the skin, and HE staining was consistent with the morphological manifestations of choriocarcinoma cells; mice in group B were tested with IVIS and showed single or multiple solid tumor masses in the body , HE staining showed that the lung tissues had tumor cell infiltration in varying degrees. On the 14th day of vaccination, the β-HCG level of the experimental group was significantly higher than that of the control group (P<0.05); the β-HCG level of group B was significantly higher than that of group A (P<0.05).

　　Conclusion: SCID beige mice injected with JAR cells subcutaneously/tail vein can successfully construct a pathological model of human choriocarcinoma transplantation tumor. This model can simulate clinical choriocarcinoma epithelial solid tumor characteristics and lung metastasis characteristics, and is a study on the pathogenesis of human choriocarcinoma A good model of mechanism and experimental treatment.