肝损伤及脑梗塞,动物造模 z-bio 2021-05-18 452

　　Background: In the field of pathology and pharmacodynamics, the demand for disease animal models is increasing. However, one of the main technical difficulties is the long-term non-invasive study of the same animal. Micro-CT is a very effective device for diagnostic imaging of laboratory animals. However, the contrast of soft tissues (especially the brain) is not enough to complete a detailed study. In this article, we will use the LCT200 to scan and image liver damage and stroke. The results show that the device can obtain good soft tissue images.

　　Method: Contrast-enhanced CT (CECT) study was carried out on mouse models of cerebral infarction and liver ischemia, and the pathological state of the model animals was changed by quantitative analysis of the amount of damage for 2 consecutive weeks. the study. We also compared micro-CT and 11.7-T micro-MRI images of stroke rats and ischemic mice to further confirm the diagnosis of CECT through pathological analysis. Conclusion: This study shows that the CECT scanning method is very effective for qualitative and quantitative research on the tissues/organs of rodents (including the brain). At the same time, this method is also very suitable for long-term research.

　　Main findings: In the cerebral infarction model, the vascular permeability increased significantly from 3 days to 1 week after the start of the operation. This change can be confirmed by Evans blue staining. Quantitative data on the degree of liver damage show that mice of different genotypes have different recovery capabilities. Comparing CT and MRI images of the same stroke or stroke mouse can prove the accuracy of volume measurement and the spatial resolution and contrast resolution of CT similar to MRI. At the same time, the CT imaging results are consistent with the pathological analysis data.