内毒素血症大鼠,动物造模 z-bio 2021-05-18 486

　　Objective: To analyze the changes of miRNA expression profile in myocardial tissue of rats with endotoxin, also known as lipopolysaccharemia, and to explore the role of miRNA in endotoxemia myocardial injury.

　　Method: 20 male SD rats were randomly divided into control group (n=10) and LPS group (n=10). The LPS group was intraperitoneally injected with LPS 10 mg/kg, and the control group was intraperitoneally injected with the same amount of normal saline. Rats were sacrificed by cervical dislocation after 24 hours, and myocardial tissue was taken. Real-time PCR was used to detect the expression levels of TLR4, TNF-α, and IL-1β, and the ultrastructural changes were observed by transmission electron microscopy. The miRNA chip screens the differentially expressed miRNAs in myocardial tissues, and Real-time PCR verifies the actual expression levels of candidate miRNAs.

　　Results: The expressions of TLR4, TNF-α, IL-1β in myocardial tissue of rats in the LPS group were significantly increased, cytoplasmic vacuoles occurred in myocardial cells, mitochondrial edema, structural destruction and other ultrastructural changes. Chip technology and Real-time PCR confirmed that miR-194-3p, miR-344a-3p, miR-465-3p, miR-501-5p, miR-3596c, miR-185-3p, miR in the myocardial tissue of rats in the LPS group -877 was significantly up-regulated, miR-208b-3p, miR-547-3p, miR-141-3p, miR-28-5p, miR-3585-5p were significantly down-regulated.

　　Conclusion: These differentially expressed miRNAs found in the myocardial tissue of endotoxemia rats may be related to the occurrence of myocardial damage.