小鼠脾脏间充质干细胞,动物造模 z-bio 2021-05-20 460

　　Objective: To establish an effective and reliable strategy for isolating and expanding mouse splenic mesenchymal stem cells by tissue block culture method.

　　Method: The mouse spleen tissue was completely ground under aseptic conditions, the spleen matrix tissue was obtained through collagenase digestion, and the digested tissue block was planted in a culture flask, and the morphology of the cells transferred from the tissue block was observed. The phenotype was analyzed by flow cytometry to induce bone and fat differentiation.

　　Result: A large number of spindle-shaped fiber cells migrated out of the digested spleen tissue mass. The results of flow cytometry showed that CD29, CD44, Sca-1, and CD105 were highly expressed, and CD11b was low. CD34, CD45 and Ia. The results of the multi-directional differentiation experiment showed that the cells migrated from the mouse spleen interstitial tissue have good adipogenesis and bone formation and differentiation capabilities.

　　Conclusion: The tissue blocking method can isolate and culture mouse spleen mesenchymal stem cells. The obtained cells have high purity and strong differentiation potential, which provides favorable conditions for carrying out related research and provides a cell model.