清醒状态下尾静脉注射,动物造模 z-bio 2021-05-20 334

　　Objective: To introduce the surgical skills and experience of rapid tail vein injection in awake rats to improve the success rate of the operation.

　　Method: The self-made rat tight-fitting jacket is combined with the operator's light pressing method to immobilize the rat. One aids in fixation, the other aids in puncture, and the other injects drugs. Minimize the damage of the rat's tail and the rat's reaction during the injection. It is necessary to strictly adjust the needle tip and the number of punctures, limit the maximum dose and injection rate at a time, and take the method of separating the needle from the injection site to avoid drugs. Pollution and waste.

　　Result: This method is fast, reliable, stable, reproducible and highly compatible with animals.

　　Conclusion: This method avoids drug waste and pollution. This method is particularly recommended for experiments using expensive drugs in the tail vein.