P-选择素缺失,动物造模 z-bio 2021-05-20 786

　　Objective: To study the effect of deletion of P-selectin gene (P-selectin) on physiological characteristics of mice.

　　Method: P-selectin knockout (PKO) mice and C57 mice were raised in an SPF environment and identified as pure PKO mice through genetic identification. Observe the activity and reproductive status of PKO mice, check blood routine, and determine the effect of P-selectin gene knockout on the general condition of mice. The mice were sacrificed at the 6th week and the 18th week, and stained with eosin and hematoxylin (HE) to observe the effect of P-selectin knockout on the structure of various organs and tissues of the mice.

　　Result: The reproductive status of PKO mice is similar to that of normal C57 mice, and the blood routine is normal. HE staining showed that the tissue structure of liver, spleen, lung and kidney of PKO mice was relatively normal.

　　Conclusion: The lack of P-selectin will not affect the reproduction and blood of mice, and has no obvious effect on important tissues or organs.