The influence of dietary intervention on the intestinal flora and health has become a research focus in the field of medical and health. From a development perspective, the intestinal flora may be an important target for studying the role of diet, health food and traditional Chinese in the preparation of human health medicines. However, the complexity of intestinal flora requires high standards of animal models to study its relationship with dietary interference. Bacterial-free animals in biomedicine and humans will not carry live microorganisms and parasites inside or outside the body. Disease and health. On the other hand, it has specific uses and research, and is the most used basic animal model in the study of intestinal microbiota. The use of sterile animals to construct human-derived plants and animals is a common method to study the relationship between diet, plants and animals and health. This article mainly summarizes the research and application of sterile bacterial animals in the construction of human-derived flora, and the influence of dietary intervention on the intestinal flora.