动物造模,自然衰老,免疫系统 z-bio 2022-01-14 424

　　Objective: To observe the changes in the structure and function of the immune system of naturally aging SD rats, and to explore its possible mechanism?

　　Method: Have you raised 60 SD rats in an SPF environment? That is 6? 12? 18? 24 months old. Do Anle10 animals use lymphocyte transformation and NK cell killing tests to determine lymphocyte function and perform immunofluorescence to detect peripheral blood lymphocyte subsets? The CBA method is used to detect serum cytokine content, and the colorimetric method is used to detect serum superoxide dismutase (SOD). Malondialdehyde (MDA)? Histopathological observation of thymus and spleen?

　　Result: Will the transformation ability of rat lymphocytes decrease with age? Will CD8 positive T cells decrease? Splenocytes target the content of NK-6 and IL-10, SOD decreases, MDA increases, thymus organ coefficient decreases, thymus decreases, splenic sinus expands, lymphoid follicles atrophy, and extramedullary hematopoiesis increases. The 8-OHdG + cells in the spleen are mainly located in red blood cells. Are the positive cells in the thymus mainly in the medulla, and will the positive cells gradually increase from the cortex to the medulla?

　　Conclusion: The degeneration of the immune system related to age provides an experimental basis for the immune damage theory of aging.