树鼩腺病毒,荧光定量PCR,动物造模 z-bio 2021-05-21 290

　　Objective: To establish a real-time fluorescent quantitative PCR detection method for tree shrew adenovirus to quickly and accurately detect tree shrew adenovirus.

　　Method: Design and synthesize specific primers based on the conserved sequence at the 3'end of the TAV genome. Use the recombinant plasmid containing the target gene fragment as a standard to draw a standard curve to establish TAVTaμMan real-time fluorescence. Quantitative PCR detection method.

　　Result: The established TAV real-time fluorescent quantitative PCR detection method is highly specific and has no cross-reactivity with trees and other common viruses. The minimum detection limit of this method is 3.7 copies per microliter, and the sensitivity is strong; the relevant detection factor R2 is 0.998, the amplification efficiency is 95.7%, the amplification results have a good linear relationship, and the reproducible detection effect is good. .

　　Conclusion: We have successfully established a real-time fluorescent quantitative PCR detection method for TAVTAμMan. It is sensitive, reproducible, and can be used for early and rapid detection of TAV infection.