系统性红斑狼疮,动物造模 z-bio 2022-01-14 1205

　　Objective: To explore the immune system of MRL/lpr mice with systemic lupus erythematosus of different ages, and to provide evidence for the study of its etiology.

　　Method: In this study, a 3-month-old SPF level was randomly selected. Have you measured organ mass and calculated organ coefficients in 10 5-month-old, 6-month-old MRL/lpr female mice and 10 SPF-grade 3-month-old wild-type C57 female mice? Use the ELISA method. Using flow cytometry, serum anti-double-stranded DNA antibody (ds? DNA)? To detect the CD3 cell content of spleen lymphocytes and CD4, spleen interleukin IL? 2? IL? Four? IL? 17 and tumor necrosis factor TNF? What is the ratio of α/CD8 cells?

　　Results: Compared with wild-type C57 mice aged 3 to 6 months, in MRL/lpr mice aged 3 to 6 months, the spleen coefficient, dsDNA antibody concentration, IL 2 and TNFα levels were significantly increased ( P \u003c0.05), the MRL/level of IL-17 of 5-6 months old compared with 3-month-old MRL/lpr mice. Lpr mice are significantly reduced (P\u003c0.05 or P\u003c0.01); other indicators of MRL/lpr mice also vary from month to month. Is there a significant difference between ages? The concentration of splenic lymphocytes in 6-month-old CD3 MRL/lpr mice was significantly lower than that of 3-month-old C57 mice (P\u003c0.01). As the age of the moon increases, the ratio of CD3 and D4/CD8 in MRL/lpr mice tends to decrease, but is the difference not significant?

　　Conclusion: The changes of all indicators indicate that 3-month-old lupus erythematosus MRL/lpr mice have a certain degree of immune damage and injury.