ＩｇＧ⁃ＨＲＰ标记抗体,动物造模 z-bio 2021-09-26 553

　　Purpose: Purify IgG antibody from Mar monkey serum, prepare rabbit anti-Mar monkey antiserum, and purify it with antiserum, HRP (horseradish peroxidase) labeled rabbit anti-Mar monkey IgG.

　　Method: HiTrapTM protein G affinity chromatography to purify mar monkey and rabbit anti-mar monkey serum IgG, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS? PAGE) for purity identification, immune agar double diffusion method (double immunization ) Rabbit anti-rabbit anti-monkey IgG? The modified "simple sodium periodate labeling method" was used to prepare mo monkey antiserum titers. Prepared HRP-labeled antibody. Enzyme-linked immunosorbent assay (ELISA) and Western blotting (Western blotting) rabbit anti-monkey IgG? The HRP-labeled antibody is used to determine the working concentration and perform specific identification.

　　Results: Purified IgG from mar monkey and rabbit anti-mar monkey sera were more than 95% and 97% pure, respectively, and the titer of rabbit anti-mar monkey IgG antiserum was 1:64. ELISA and Western blot detection of rabbit anti-mar monkey IgG? When the reference working concentration was 1:256,000 and 1:15,000, the HRP enzyme-labeled antibody was identified with clear specificity.

　　Conclusion: Does Marmoset IgG include the specificity and concentration of ELISA and Western blotting? The preparation and pre-identification of HRP-labeled antibodies reserve resources for the immunological and molecular immunological detection system of mar pathogens.