动物模型,高血压 z-bo 2020-08-06 557

　　(1) Reproduction method The adult experimental rats were conventionally anesthetized and made a midline abdominal incision, and the left kidney was removed. After the operation, the rats were injected subcutaneously with 50 mg/kg body weight of deoxycorticosterone acetate (DOC) dissolved in olive oil for 5 days a week for 5 weeks, while drinking 1% NaCl solution instead of drinking water. Drink regular water after stopping the administration. After 1 week of administration, blood pressure began to rise, and after 5 weeks, 70% of the rats developed persistent hypertension, and those with systolic blood pressure greater than 160mmHg (21.3kPa) were used as successful models for the test.

　　(2) Model characteristics: The subcutaneous injection site should be frequently replaced and disinfected. Rats with DOC alone are not easy to cause stable high blood pressure. Removal of one kidney and drinking 1% NaCl can cause persistent high blood pressure. When preparing hypertension models, NaCl is often used as a promoting factor for the formation of hypertension. Some scholars have shown that rats are given high-salt feed (containing 4% NaCl) for 18 weeks from childhood (3 weeks) to form chronic hypertension, called salt hypertension.

　　(3) Comparative medicine The DOC salt-induced hypertension model is one of the more commonly used hypertension models. It is easy to prepare and has relatively stable hypertension, and its response to antihypertensive drugs is more consistent with that of hypertensive patients. The salt-induced hypertension model does not require surgical treatment first, and does not affect other physiological functions of the rat, but the modeling time is longer.