动物造模,原发免疫性血小板减少性紫癜热盛模型 z-bio 2021-05-24 486

　　OBJECTIVE: To establish a peripheral blood thrombocytopenia model that conforms to the traditional Chinese medicine "Heatchen syndrome" and primary immune thrombocytopenic purpura.

　　Methods: Multiple injections of 20% dry yeast suspension into the back of SD rats, intraperitoneal injection of 1:4 dilution of rabbit anti-SD rat platelet serum (APS), primary immune thrombocytopenic purpura to establish a rat disease fever model . Then, observe the TCM syndrome characteristics, blood image, bone marrow image and 5-hydroxytryptamine (5-HT) level of the temperature regulation center of the thalamus of the tested rats.

　　Result: 2-6 hours after injection, the body temperature and daily fluid intake of rats in the model group increased significantly. Toe purpura appeared on the 4th day of the model, and intestinal mucosal hemorrhage appeared on the 30th day of the model (P\u003c0.05); the number of platelets decreased significantly, and the number of megakaryocytes in the bone marrow increased significantly. Decrease (P\u003c0.05); the 5-HT level of the brain temperature regulation center increased significantly (P\u003c0.05).

　　Conclusion: The rat model of primary immune thrombocytopenic purpura combined with diseases and syndromes is essentially the pathology of primary immune thrombocytopenic purpura caused by "purpura" rats "fever", reflecting its characteristics .