动物造模,慢性心肌缺血小型猪模型 z-bio 2022-02-06 319

　　Objective: Vitamin D3 (VD3) and isoproterenol were injected intravenously to establish a piglet model of high-fat chronic myocardial ischemia.

　　Method: 15 male Bamaminiature pigs were randomly divided into myocardial ischemia model (CMI) group, normal control group (Ctr), high fat (HFC) group and sputum congestive inverted syndrome (CMI) group. Using the normal diet of the Ctr group, the high-fat diet of the HFC group, the high-fat diet of the CMI group, and intravenous injection of VD3 and isoproterenol, a chronic myocardial ischemia model of sputum and congestion syndrome was established. . The model is modeled continuously. The animals were weighed separately and the 24-week ECG was measured to establish a model and evaluate the pathological process. , Activity, blood lipids, myocardial enzymes, hemorheology, inflammation, cardiac index (CI), myocardial ischemia (MIS), sputum and congestion.

　　Results: Compared with the Ctr group, the HFC group weight, heart rate (HR), total cholesterol (TC), low density lipoprotein (LDL-C), high density lipoprotein (HDL-C), atherosclerosis and atherosclerosis The sclerosis index (AI), low, medium, and high-cut whole blood and low viscosity, lactic acid electrolysis time (EPT), sensitive C-reactive protein (hs-CRP), interleukin 6 (IL-) 6) levels were significantly increased ( P\u003c 0.05, P (u003c0.01); CMI group weight, HR, 24-hour total ST-segment increase (∑ST), average ST-segment increase within 24 hours (P_u003c 0.05, P\u003c0.01) ST_average), activity , TC, triglyceride (TG), LDL-C, HDL-C, AI, creatine kinase (CK), lactate dehydrogenase (LDH), troponin 1 (cTn-1), low, medium, High-cut whole blood and low viscosity, EPT, Casson viscosity (CV), hs-CRP, IL-6, CI and MIS all significantly increased (P\u003c0.05, P\u003c0.01) and adiponectin ( APN). ) The level is significantly reduced (P\u003c0 .05). Compared with the HFC group, the low, medium and high cut whole blood viscosity, EPT, CI and MIS of the AI, CK, LDH, cTn-1, and CMI group were significantly higher than those of the HFC group (P\u003c0.05, P\(u003c0 .01), the level of APN was significantly lower than that of the HFC group (P\u003c0.05). Correlation analysis showed that MIS and TC, LDL-C, AI, CK, LDH, cTn-1, APN, high, medium, and low-cut complete Blood viscosity, EPT, CV, hs-CRP, IL-6 correlation (P linear regression analysis shows that varicose veins and blood stasis are TC, LDL-C, AI, CK, LDH, cTn-1, APN., EPT, CV, hs-CRP and IL-6 (P\u003c0.01), and linear stepwise regression analysis showed that the development of hemorrhoids and blood stasis are closely related to TC, CK and IL-6 (P\u003c0.01).

　　Conclusion: High-fat feeding and injection of VD3 and isoproterenol can successfully establish a small model of chronic myocardial ischemia with stasis and congestion syndrome. Lipid metabolism, hematology, myocardial enzyme metabolism and inflammation can be used as blood stasis and inflammation. Blood stasis syndrome. The objective biochemical material basis of blood stasis reciprocal congestion syndrome, these changes are the traditional Chinese medicine theory "blood stasis reciprocity, the combination of blood stasis and toxins.