动物造模,高血流性肺动脉高压模型建立 z-bio 2021-10-09 739

　　Objective: To establish a rat model of hypertensive pulmonary hypertension and anastomosis of the common carotid artery and external jugular vein.

　　Method: Forty-five healthy male SD rats were divided into shunt group, ligation group and sham operation group (n = 15). After 12 weeks, the overall condition of the rat will be confirmed, and an echocardiogram will be performed to observe the heart, cardiac output, and patency status. Check the right ventricular systolic pressure with a right heart catheter, and analyze blood gas to calculate pulmonary circulation/body. Circulating blood flow ratio (Qp/Qs); right ventricular hypertrophy index; HE staining of lung tissue and EVG staining of elastic fibers. Statistical data uses SPSS 16.0 software, and one-way ANOVA is performed on each set of data.

　　Result: There was no significant difference in weight gain between the two groups, and the anastomotic patency rate was 84.6%. Compared with the sham operation group and the ligation group, the heart of the rats in the shunt group was significantly enlarged, and the cardiac output increased significantly ((309.8±33.1) mL/min·Kg to (245.6±31.9) mL./min·Kg. (240.8±30.9) mL/min·kg, P\u003c0.05]; the Qp/Qs of the shunt group was 2.16±0.38, and the right ventricular systolic blood pressure was (35.8±4.9) mmHg. It has risen. The right ventricular hypertrophy index in the shunt group It was 0.3263±0.0342, which was significantly higher than the other two groups. Compared with the sham operation group, the midline thickness of the pulmonary artery in the shunt group was significantly increased [(22.3±1.7)% to (10.6±1.7)%, P\u003c0.05].

　　Conclusion: Anastomosis of the common carotid artery and external jugular vein in rats can establish an excellent model of high pulmonary blood flow and pulmonary hypertension.