动物造模,急性硬膜下血肿模型 z-bio 2021-12-07 346

　　Objective: To study the effect of different amounts of autologous blood on the stability of rat subdural hematoma model.

　　Method: The rats were randomly divided into sham operation group and 300μL, 500μL, 700μL model group (ie 300μL, 500μL, 700μL autologous subdural injection). Six rats in each group were collected on the second, fourth, sixth, eighth, tenth, and 14th day after the operation, blood was collected from the abdominal aorta, brain tissue was collected, and the size was determined using the ELISA method. The content of NSE and S100B protein in rat plasma.

　　Results: Compared with the sham operation group, the blood NSE of the 300μL group increased significantly on the 2nd and 4th day (P0.05), while the NSE content of the 500μL and 700μL groups were both 2, 6, 8, 10 And 14 days. Significant increase (P0.05). The blood S100B protein content in the 300μL group was higher on the 4th day (P0.05), indicating that the hematoma can subside spontaneously and the damage can be repaired spontaneously. The S100B protein content in the 500μL and 700μL groups was always high (P\u003c0.05).

　　Conclusion: Compared with the 300μL and 700μL groups, the 500μL group is more stable in the acute subdural hematoma model, and is more suitable for experimental studies of acute subdural hematoma in rats.