动物模型,高血压 z-bo 2020-08-06 473

　　Unconditioned stimuli (injection of epinephrine, angiotensin or electrical stimulation, etc.) are repeatedly combined with conditioned stimuli to induce hypertension, which can be performed in dogs, rats, rabbits and other animals.

　　(1) Reproduction method 12-week-old male Wistar rats were given stress stimulation before the experiment, and those who were quick to escape, scream, raise their tails, and gasp were selected as the model animals. The compound stimulus of foot electric shock combined with noise is selected. The intensity of the stimulus pulse power source shall not cause damage but cause a strong reaction. The current is 0.1～0.8mA, the pulse interval is 20～150ms, the wave width is 2～20ms, and the stimulation voltage output is 75～150V (adjustable). The frame and wall of the experimental mouse box are plexiglass panels, 22cm×22cm×16cm. The bottom of the box is made of copper pipes with a diameter of 3mm. The copper pipes are spaced 1cm apart and connected to the stressor. The noise source is placed in front of the stress box, not in contact with the rat box. The rats in the experimental group received stress stimulation once every day from 8:00 to 10:00 in the morning and from 2:00 to 4:00 in the afternoon for 15 days. The blood pressure and heart rate were measured by the tail cuff method one day before the stress stimulus and the 3rd, 6th, 9th, 12th, and 15th day after the stress stimulus, and the behavior changes were observed. Prepare models of different stress times, with periods of stress 3, 6, 9, 12, and 15 days. During the stress process, the blood pressure of rats increased significantly, and it rose rapidly to (156.4±14.0) mmHg (1mmHg=0.133 kPa) within 6 days, which was the highest blood pressure after stress, and was (150.5±12.0) mmHg on the 9th day . After that, it remained relatively stable at this high level, and it was still (152.4 ± 11.0) mmHg on the 15th day. Heart rate increased significantly from (406.0±18.0) beats/min to (434.6±20.0 beats)/min on the 3rd day after stress, and increased to (478±21.0 beats)/min on the 6th day, and reached ( 463.0±17.0 times)/min.

　　(2) Model characteristics The incidence of hypertension animal models induced by this method is higher than that of the same type of experimental stressors, and the time required for stress stimulation is short, and the experimental stressors used are better than similar ones. Stimulus.

　　(3) Comparative medicine uses electric shocks and random noise signals as conditioned stimuli, which greatly increases the psychological component of stress, facilitates the psychological response of animals, and causes animal hypertension, which is similar to human stress hypertension. Repeatable and quantifiable procedures.