二乙基亚硝胺,诱发性肝癌模型,动物造模 z-bio 2021-05-24 782

　　Liver cancer is one of the main threats to human health and ranks second in tumor mortality. The development of primary liver cancer is the result of multiple causes and multiple stages of hepatitis-cirrhosis-early liver cancer-advanced liver cancer-liver cancer metastasis. During this period, various virulence factors may be involved, such as viral hepatitis and alcoholic cirrhosis. The average interval between the first viral infection and the clinical examination for liver cancer is usually 20 to 40 years. The rodent mouse model is a widely used animal model of liver cancer. In the induced liver cancer model, DEN, dimethylnitrosamine, carbon tetrachloride (carbon tetrachloride, CCl4) and other substances are commonly used carcinogenic inducers. Among them, DEN is an organic substance derived from N-nitroso, which can stably induce tumors in various tissues such as the liver, lung, stomach, and hematopoietic system. The DEN model can cause severe liver damage, such as carcinogenicity, mutagenicity and teratogenicity. The most prominent symptoms are neutrophil infiltration, lobular hemorrhagic necrosis, abnormal liver cell division, bile duct hyperplasia and fibrosis. cancer. However, studies have shown that nitrosamines have different effects on mice with different genetic backgrounds and genders. Differences in administration time and dosage may also affect the results of the experiment. Therefore, the purpose of this article is to summarize previous literature studies, propose procedures for using DEN in mice, and establish an effective DEN mouse liver cancer model.