动物模型,动脉粥样硬化 z-bo 2020-08-06 668

　　(1) Reproduction method New Zealand rabbits with a weight of 2.0～2.5kg and an age of 3～4 months, after observation for 1 week, give feed containing 1.5% cholesterol 80g/(kg·d), take water freely, and feed 12 week. After the animals were fed for 12 weeks, their serum TC and LDL-C levels were significantly increased, and a large amount of yellow and white lipids protruded from the aortic wall to the lumen and connected into a sheet, from the proximal to the distal end of the aorta , The plaque gradually reduced, the plaque/intima area ratio was (32.48±3.31)%; the vascular intima was significantly thickened, the plaque bulged, and contained a large number of foam cells; the number of macrophages increased significantly, and more Located in the lower middle part of the intimal plaque. If another high-fat formula: 0.5% cholesterol, 5% lard, 94.5% basic feed, fed for 6 to 8 weeks, the incidence of aortic plaque can reach 80% to 100%, and serum cholesterol will be significantly increased. Add 15% egg yolk powder, 0.5% cholesterol and 5% lard to the feed. After 3 weeks, the cholesterol in the feed is subtracted and fed for another 3 weeks to make the incidence of aortic plaque reach 100%.

　　(2) Features of the model This model is made by feeding rabbits with high fat and high cholesterol. After the model is formed, blood lipids change significantly. It is an atherosclerosis model with hyperlipidemia. Experimental animals are easy to obtain, cheap, simple to operate, and highly sensitive to disease-making diets. The appropriate dose of cholesterol is added to the modeled feed, so that the model can be successfully modeled in a short period of time, and this type of model is avoided The common shortcoming of long modeling time is that rabbit modeling is better than mouse, rabbit, pig, dog, monkey and other experimental animals.

　　(3) Comparative medical model animal rabbits given high-fat and high-cholesterol diets can develop obvious atherosclerosis after 3 to 4 months, and its pathological changes are similar to those in humans; but the ester metabolism of rabbits is similar to that of humans. The ester metabolism is quite different; the anatomical distribution of the lesions is mainly the thoracic artery, the coronary artery lesions are mostly present in the small arteries of the heart, while the humans mainly occur in the large branches of the coronary arteries.