动物造模,中动脉栓塞脑梗死模型 z-bio 2021-10-26 367

　　Objective: To explore the feasibility of using laser Doppler blood flow monitoring technology to establish a stable model of cerebral infarction of middle cerebral artery in rats.

　　Method: 16 SD male rats were randomly divided into 2 groups: 8 experimental groups and 8 control groups. The experimental group judged that the cerebral blood flow decreased to 30% of the basic value during the model making process. The control group did not monitor the cerebral blood flow and inserted a nylon cord at a depth of 1.8 cm. It is considered a successful model creation. The nerve injury severity score (mNSS) was performed 24 hours before and after the model. The rats were sacrificed 24 hours after the model. The brain was collected and stained with 2,3,5-triphenyltetrazolium chloride (TTC) to calculate the brain infarction.

　　Result: 8 rats in the experimental group all developed typical hemiplegia symptoms 24 hours after the model. The mNSS score was stable at 10-13 points, and the infarct volume stability and uniformity were good (37.5±3.9). %. The mNSS scores of eight rats in the control group were unstable. The mNSS scores of the five rats ranged from 10 to 13 points. The cerebral infarction damage of 5 rats was similar to that of the experimental group, but 3 rats had cerebral infarction. It is significantly smaller than the experimental group (P\u003c0.05). The model success rate of the experimental group was 100%, and the model success rate of the control group was 62.5% (P\u003c0.05).

　　Conclusion: Laser Doppler blood flow monitoring technology can significantly improve the success rate, stability and uniformity of the rat MCAO model.