动物造模,周期性麻痹模型 z-bio 2021-11-23 553

　　Objective: To establish and evaluate the hypokalemic periodic paralysis model of hyperthyroidism by using gene knock-in CaV1.1-R528H mice.

　　Methods: 8-week-old gene knock-in CaV1.1-R528H male mice and 36 8-week-old wild-type C57BL/6J male mice, using 3 factors, 2 levels, 2×2×2 factor planning method, body weight was divided according to the random principle Eight groups (three mutant factors, thyroxine and insulin, with or without two levels). Among them, mice in the thyroxine treatment group were continuously injected intraperitoneally with levothyroxine sodium weighing 350 μg/kg for 12 days to treat hyperthyroidism. After the last dose, a short-acting intraperitoneal injection was given to the insulin treatment group. Before injection (0 minutes) and after injection (30, 60 minutes), the insulin and serum potassium of 0.8 U/kg body weight were detected and recorded in each group of mice.

　　Results: (1) Hyperthyroidism mice showed hypersensitivity, hypersensitivity and dry clothes, and compared with the control group, the amount of food and drinking water increased significantly, and the weight increased, it was too late. Thyroid function test showed that T3 and T4 were significantly higher than the corresponding control group, and TSH was significantly lower than the corresponding control group, indicating a significant difference (P\u003c0.05). (2) When thyroxine or insulin was used alone, there was no significant difference in serum potassium between the mutant group and the wild group. However, after insulin treatment under hyperthyroidism, the mutant group and the wild group were treated simultaneously at 30 minutes and 60 minutes. The serum potassium level of the group was significantly lower than that of the wild-type group (P\u003c0.05). (3) The main function and interaction: the mutation factor or thyroxine factor alone does not affect the serum potassium, only insulin affects the decrease of serum potassium (P\u003c0.05); the sudden change between the thyroxine factor and the mutation factor and the insulin factor Interaction (P\u003c0). 05); There is no interaction between thyroxine factor and insulin factor.

　　Conclusion: (1) The treatment of hyperthyroidism is successful. (2) Gene knock-in CaV1.1-R528H mice successfully established a hypokalemic hypokalemic periodic paralysis model.