动物造模,Hr基因敲除小鼠模型 z-bio 2022-01-31 411

　　Objective: To establish knockout mice and study the function of hairless gene (Hr) in detail.

　　Method: Use transcriptional activator-like effector nuclease (TALEN) technology to prepare Hr gene knockout mice, observe the hair growth and development of gene knockout mice, and microdissection paraffin sections of skin tissue. Analyze the results. As a result, the first mouse to delete 2 bases in the coding region of the 86-87 Hr gene produced a TGA stop codon. Positive offspring are obtained by mating with wild-type mice and mating with the same pups. The second-generation mice began to lose their hair on the 14th day, lost their hair around the 30th day, and remained hairless for the rest of their lives. Histological observation of the skin showed that cysts of various sizes were formed in the dermis.

　　The results of the study showed that the successfully established Hr gene knockout mice showed abnormal hair growth. The conclusion proves the close relationship between hairless gene and hair growth and development, and provides an excellent animal model for studying the exact function of Hr gene.