动物造模,2型糖尿病小鼠模型 z-bio 2022-01-31 905

　　Objective: To compare the differences in glucose metabolism, insulin resistance and inflammation between spontaneous and induced type 2 diabetes mouse models.

　　Method: The animals were divided into a normal control group, a provocative model group and a spontaneous model group, with 10 animals in each group. Normal control group: normal 11-week-old C57BL/6J mice; induction model group: use a combination of high-fat diet and STZ to induce 11-week-old C57BL/6J mice to establish a diabetes model. Spontaneous model group: select 8-week-old C57BL/ KsJ-db/db mice; monitor the body weight, fasting blood glucose and glucose tolerance changes, FINS, AUC, HOMA-IR, and detect the changes of TNF-α in each group within 8 weeks. Before and after the experiment, the contents of IL-18, IL-1β and INF-γ; 8 weeks later, I collected liver, kidney, pancreas, testis, skeletal muscle and other tissues, but sacrificed the animals for pathological analysis.

　　Result: During the experiment, the body weight of the spontaneous model group continued to increase, but the body weight of the model group was induced to decrease significantly. The blood glucose level of the induced model group decreased after 8 weeks, but the blood glucose level of the spontaneous model group remained unchanged. Hyperglycemia is stable, FINS in the provocation model increased in the spontaneous model group, AUC and HOMA-IR decreased significantly, while FINS in the spontaneous model group did not change significantly. Both AUC and HOMA-IR increased significantly. After the model was established, the levels of serum inflammation-inducing factors (TNF-α, IL-18, IL-1β, INF-γ) were higher than those of the normal control group. At the 8th week, the pro-inflammatory factor content in the stimulating model group decreased, and the pro-inflammatory factor content in the spontaneous model group increased significantly. There was obvious inflammatory damage in the pancreas and other tissues of the two model mice.

　　Conclusion: After 8 weeks, the abnormal glucose metabolism and inflammation of the spontaneous type 2 diabetes model gradually increase, and the symptoms of hyperglycemia can be maintained for a long time. After 8 weeks of growth, glucose metabolism and inflammation in the induced type 2 diabetes model were partially relieved.