动物造模,心梗模型 z-bio 2021-05-25 756

　　OBJECTIVE: To establish a myocardial infarction model and clinically important model indicators in goose Potentilla piglets, and to explore the correlation between gene expression in the marginal zone of myocardial infarction and metabolic compensation.

　　Method: Choose 13 mincemeat Lingcai mini pigs and randomly divide them into groups. Five of them were in the sham operation control group, five were in the myocardial infarction operation group, and the other three were in the normal control group without surgery. After anesthesia, the left anterior coronary artery was permanently ligated with the main branch of the descending branch near the apex, resulting in myocardial ischemia and infarction. Four weeks after the operation, cardiac ultrasound, blood sampling and serum biochemical analysis were performed. After euthanasia, the area around the infarction was collected for pathology and genetic chip analysis.

　　Result: The ultrasound image showed that the area of myocardial infarction was clear. The data showed that compared with sham operation, the left ventricular drainage rate and short axis shortening rate of the myocardial infarction operation group were reduced by 32% and 40%, respectively. Surgery group; there was increased serum aspartate aminotransferase, serum bilirubin, lactate dehydrogenase, etc.; pathological sections were destroyed and dissolved by myocardial fibers, connective tissues around the connective tissue proliferated, and neutrophils and lymphocytes increased; genes Expression profile analysis revealed two pathways that regulate post-MI remodeling and histopathology, three pathways of inflammation and immunoregulation, as well as fat and protein pathways, indicating that gene expression has occurred in the eight pathways of carbohydrate energy metabolism. Changing.

　　Conclusion: We have successfully established a model of myocardial infarction in Potentilan serina mini-pigs, which has few coronary branches, is easy to operate and stable. The characteristics of this model may be consistent with clinical indicators. The model infarct marginal zone and the clinical metabolic compensation show the same trend of change, and provide characteristic changes for experimental animal models and the reference value of myocardial infarct marginal zone to study the mechanism of myocardial infarction.