动物造模,胚绒膜尿囊膜移植瘤模型 z-bio 2021-05-26 910

　　Objective: By comparing the growth of three different cancer cells in chicken chorionic villi (CAM), screening the best transplanted cancer cell lines, establishing a transplanted tumor model, and observing its biological characteristics.

　　Methods: Human lung cancer A549 strain, tongue squamous cell carcinoma TCA8113 strain and human liver cancer QGY7703 strain were respectively inoculated into 7-day-old chicken embryo CAM. The survival rate of each chicken embryo was tested for tumor strain, tumor formation rate and induced blood vessel Generate other indicators, observe and screen the growth characteristics of transplanted tumor models.

　　Result: Compared with human lung cancer A549 and liver cancer QGY7703 vaccination group, tongue squamous cell carcinoma TCA8113 has the highest tumor incidence (P\u003c0.05), and is the best cancer cell line for transplantation. The best number. The number of cancer cells inoculated is 8.0×106/cell, the best tumor growth cycle after inoculation is 4-8 days, and the best experimental time is 7 days after inoculation.

　　Conclusion: The screening and establishment of TCA-CAM transplantation tumor model of tongue cancer squamous cell carcinoma is very useful for detailed study of tumor growth biological characteristics, angiogenesis mechanism, invasion and metastasis mechanism, and anti-tumor screening. We provide experimental animal models.