动物造模,急性肝功能衰竭模型 z-bio 2021-12-21 445

　　Objective: To study the effect of different ratios of hepatectomy doses on acute liver failure in Bama mini-pigs, and to establish a suitable model to provide a suitable method for acute liver failure after hepatectomy in most mini-pigs.

　　Method: Perform 75%, 85% and 95% liver resections respectively, and check the remaining liver by CT and record the survival status. Collect blood regularly before surgery. 1, 3, 5 days, 1, 2 and 3 weeks after the operation, HE staining was performed on the liver tissue to confirm the liver injury.

　　Result: The average survival time of 75%, 85% and 95% of hepatectomy minipigs was (19.0±5.6) days, (17.3±5.5) days and (1.3±1.5) days, respectively. The pathological scores of Bamamini pigs with different liver resection rates were (5.67±0.52), (8.17±0.82) and (8.50±0.71), respectively. The higher the liver resection rate, the higher the incidence of liver failure. Eighty-five percent of liver resections can significantly increase the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase and total bile acids.

　　Conclusion: 85% of liver resections can lead to a typical model of acute liver failure in miniature pigs.