动物造模,诱导性小型猪动脉粥样硬化模型 z-bio 2021-05-26 721

　　OBJECTIVE: To establish a model of small atherosclerosis (AS) induced by high fat and to observe the expression changes of lipoprotein-associated phospholipase A2 (Lp-PLA2) in plasma and plaques.

　　Method: Ten Gosashiyama mini-pigs were randomly divided into a normal control group (Ctr), four were fed with a regular diet, and the AS model group was fed with a high-fat diet for 24 weeks. Twenty-four weeks after modeling, venous blood was collected before fasting to detect total cholesterol (TC), low-density lipoprotein (LDL-C), high-density lipoprotein (HDL-C), triglycerides (TG) and C . The aorta was taken for reactive protein (CRP), Lp-PLA2 activity and composition changes, and oil red O staining, and the abdominal aortic vessels were taken for HE staining and IL-6 immunohistochemical staining to observe blood lipids. Changes in deposition and plaque pathology and IL-6 protein expression. We used RT-PCR and Western Blot to observe the expression of Lp-PLA2 mRNA and protein in the abdominal aorta.

　　Results: Compared with the normal control group, the weight, body mass index (BMI), TC, LDL-C, HDL-C, CRP, Lp-PLA2 activity and composition of the AS model group increased significantly (P\u003c 0.05, P\u003c0 .01) At the same time, the aortic lipid deposition was obvious (P\u003c0.01) and AS plaques were formed. The expression of Lp-PLA2 mRNA, protein and IL-6 protein in abdominal aorta tissue were significantly increased. (P\u003c0.05).

　　Conclusion: A 24-week long-term high-fat diet can induce AS in Gosashiyama miniature pigs. Lp-PLA2 plays an important role in vascular inflammation and AS plaque formation.