动物造模,阿尔茨海默病转基因模型 z-bio 2021-10-22 867

　　Purpose: The pathological changes of Tg2576 transgenic mice are similar to Alzheimer's disease (Alzheimer's disease, AD). This article dynamically studied the characteristics of the serum metabolites of Tg2576 mice at various stages of AD, and provided an early diagnosis for the clinical basis of AD metabolism.

　　Method: Collect serum samples of Tg2576 mice in the early (6 months) and final (12 months) stages of AD onset, collect 1 HNMR spectra of the samples, and collect metabolic characteristics using multivariate analysis techniques.

　　"Results: The results show that there are significant differences in serum metabolism characteristics between 6-month and 12-month-old Tg2576 and C57 mice, and Tg2576 mice have significant metabolic differences at all stages of AD. Compared with C57 mice, in the early stages of AD development, serum lactic acid, inositol, amino acids (leucine, isoleucine, alanine, etc.) levels in Tg2576 mice increased, lipids and choline, The content of phosphatidylcholine/glycerol phosphatidylcholine and betaine is increased. Glycine and glucose content decreased; at the end of AD attack, serum lactic acid, inositol and alanine content continued to increase, lipid, choline, phosphatidylcholine content continued to decrease, but alkali/glycerol phosphatidylcholine, betaine The content of glycine and glycine continues to rise and decreases. The content of glutamate and creatine initially showed a downward trend. Comparing the serum metabolites of early and late AD shows that serum lactic acid, inositol, and alanine levels are elevated in advanced disease, while lipid, choline, phosphatidylcholine, and glycerophosphatidylcholine levels are reduced. Among these metabolites, lactic acid, lipids, choline, phosphatidylcholine and glycerol phosphatidylcholine have significant changes in the early stages of AD, and are closely related to the severity of AD.

　　Conclusion: The results show that lactic acid in Tg2576 mice is positively correlated with the progression of Alzheimer's disease, while lipid, choline, phosphatidylcholine and glycerophosphatidylcholine are negatively correlated.