动物造模,胶质瘤原位移植瘤模型 z-bio 2021-05-26 694

　　Objective: To establish a stable, real-time monitoring model of glioma allograft in nude mice.

　　Methods: U251 glioma cells were infected with lentivirus containing luciferase-Luc and green fluorescent protein-GFP genes, and cell lines stably expressing GFP-Luc fluorescence were screened by flow cytometry, and fluorescent cells were screened out. Growth, migration and invasion. Through CCK-8 experiment, cell cycle experiment, Transwell tumor migration and invasion experiment, change the cell volume; use the mouse real-time imaging system to monitor tumor growth and control the tumor model of glioma. For establishment of transplantation, cells were inoculated into the caudate nucleus of nude mice. The pathological features and tumorigenicity of the cells in the brain and the brain of nude mice were evaluated by paraffin sections and HE staining.

　　Result: We successfully constructed a U251 glioma cell line and animal model that stably express GFP fluorescence and luciferase fluorescence. Lentiviral integration does not change the cell's ability to proliferate, migrate or infiltrate. This model has a medium growth cycle and high tumor incidence. , The tumor grows stably in the skull, and the HE film conforms to the characteristics of human glioma.

　　Conclusion: Compared with traditional cells, dual fluorescent labeled glioma cells further promote the experimental study of glioma animal models; the nude mouse model of U251-GFP-Luc glioma cells is human, and it has Tumor similar to tumor growth and pathological characteristics. Glioma is similar, and tumor growth can be observed in real time, making it an ideal animal model for experimental research on glioma.