动物模型,动脉粥样硬化 z-bo 2020-08-06 560

　　(1) Replication method Newborn Wistar rats are injected with 10% monosodium glutamate (MSG) subcutaneously at the dose of 4g/kg body weight on the 1, 3, 5, 7, and 9 days after birth. 5 times. Thirty days after MSG injection, the aorta was taken for electron microscopic observation. It can be seen that there are microvilli-like protrusions on the luminal surface of the endothelial cells of the aortic wall, the endothelial cell nuclei are somewhat distorted and irregular, and the subendothelial layer is not thickened; 60 days after the injection of MSG, the endothelial cells of the animal blood vessel wall degenerate and fall off, and the subendothelial layer is obviously thickened. Many vacuoles and hyperplastic collagen fibers of varying sizes, macrophages and smooth muscle cells migrate into the subendothelial layer.

　　(2) Model characteristics: This model is different from food and drug-induced As models. It destroys the arcuate nucleus of the hypothalamus by injecting monosodium glutamate, leaving aside the pathological process of the blood vessel itself, and copying the As model from the "central mechanism", which is similar Models such as long-term stimulation of the rabbit hypothalamus can cause hyperlipidemia and As formation, destroy the hypothalamus and make coronary atherosclerosis worse, and have the advantages of simple method, accurate quantification and reliable results.

　　(3) Comparing the characteristics of the animal’s histopathology after this model was established: the endothelial cells of the aortic wall were degenerated, the nucleus swelled, the subendothelial layer was thickened and vacuoles, and smooth muscle cells migrated to the subendothelial layer. These changes are consistent with the characteristic lesions of the early onset of clinical As; the substances closely related to the formation of As in the blood have also changed, total cholesterol (TC), oxidized low-density lipoprotein (OX～LDL) and lipid peroxide (LPO) ) Are all increased, while nitric oxide (NO) is decreased. These changes are very similar to the changes in As induced by drugs. This model can be used as a model to explore the formation mechanism of the central hypothalamic arcuate nucleus and As.