动物造模,大鼠动脉移植模型 z-bio 2021-10-22 451

　　Purpose: Allograft vascular disease (AV) limits the function and long-term survival of transplanted organs. This is the most pressing global issue in the field of organ transplantation. Traditional vascular suture requires good microsurgical techniques and is difficult to perform. Exploring more convenient and feasible arterial transplantation methods can provide ideas for clinical research on atrial fibrillation.

　　Method: Use an indwelling venous needle to make a self-made vascular cannula, pull the thoracic aorta of the donor rat into the cannula, turn both ends upside down, ligate and fix it to the graft, and then implant the graft. At 1, 4, and 8 weeks after modeling the abdominal aorta of the recipient, the grafted artery was used for HE staining to observe the morphology of the grafted blood vessel and measure the thickness of the endometrium.

　　Results: 38 of the 40 recipient rats survived to the required detection point, their lower limbs and tail moved freely, and urine and urine were normal. HE staining showed that the transplanted endometrium was AV. Match type. Thicken.

　　Conclusion: This method of rat artery transplantation is simple and feasible. Compared with traditional vascular suture, it significantly reduces the obstruction time of the abdominal aorta, reduces the surgical trauma to the recipient rat, and has a higher success rate. Model building and strong repeatability.