动物造模,不同程度寒颤大鼠模型 z-bio 2021-05-27 545

　　Objective: To study the temperature threshold of shivering during cryotherapy in hyperthermia rats, and to establish rat models of different degrees of shivering.

　　Method: Using 20% dry yeast suspension, heating male SD rats with a body weight (200±20) g and a basal body temperature of 36.8°C to 38.3°C. Select 40 rats that successfully developed a fever and divide them randomly. Exploratory cooling treatment was performed under the neck and arms of the hot rats for 30 minutes, using 4 groups of 10, 20, and 40 mL ice packs, with 10 animals in each group. The control group was not cooled. Observe the appearance of chills in rats and monitor the rectal temperature threshold of chills in rats.

　　Result: Within 30 minutes of cooling, the rats in the control group and the 10 mL ice pack group did not chill. Rats in the 20 mL ice pack group developed mild chills, manifested by vertical hair and head and neck tremors. The average anal temperature threshold for mild chills is 37.25°C, and the incidence of mild chills with or without upper limb tremor is 100%; the hair, head and head of the rats in the 40 mL ice pack group are upright, and The neck is severely chilled, the body and trunk tremble violently, the tail muscles become tense, the average chilly anus temperature threshold is 37.07°C, and the incidence of chills is 90%.

　　Conclusion: The use of ice packs for cooling intervention in hot rats can establish an ideal model to prevent chills, moderate chills and severe chills.