动物造模,缺氧缺血性脑损伤模型 z-bio 2021-05-27 276

　　Objective: To improve the classic Vannucci method and establish a simple and stable model of hypoxic-ischemic brain damage in neonatal rats.

　　Method: The 11-day-old newborn KM mice were divided into normal control group (N group, n=20) and ischemic hypoxia group (HIBD group, n=160). The left common carotid artery was ligated in the HIBD group. Model hypoxia under C1-C8 conditions. After modeling, the most stable modeling condition was selected by comparing the mortality of mice under each condition, the success rate of modeling and the amount of strokes stained by TTC. After modeling, the body weight growth curve was used to analyze the growth and development of the mice, and the Longa, Griptest and suspension tests were used to evaluate the neuromotor function of the mice. Use HE staining to observe the pathological changes of brain tissue.

　　Result: Newborn mice in the HIBD group were ligated to the left common carotid artery, hypoxia for 45 minutes at 8% O2 and 35°C, low mortality (8.3%) and high mold rate (47.92%). Weight gain is slow and has severe neuromotor dysfunction. The area of cerebral infarction appeared on the ligation side, accounting for (17.76±0.70)% of the total brain volume. Necrosis occurs when the neurons in the cerebral cortex and hippocampus of the ligation side are degenerated.

　　Conclusion: In this experiment, neonatal mice were used to ligate the left common carotid artery. The HIBD animal model was replicated for 45 minutes under hypoxic conditions of 8% O2 and 35°C.