动物造模,子宫内膜异位症盆腔粘连大鼠模型 z-bio 2021-12-04 371

　　Objective: To explore the methods and indicators for establishing a rat model of endometriosis with pelvic adhesions.

　　Method: Use autologous transplantation to transplant the endometrium into the rat mesentery. Eight rats were randomly selected on the 1, 3, 5, 7, 14, 21, and 28 days after the operation to observe the growth of the lesion. This status has been adopted. Use Society for Reproductive (AFS) Adhesion Grade (AFS) and Harbor Adhesion Score to simultaneously score the degree of pelvic adhesion. At the same time, score the lesion and the surrounding endometrial tissue. The HE staining was used for each group, and the adherent tissue around the lesion was collected 5, 7, 14, 21, and 28 days after surgery to determine the content of tissue plasminogen activator (tPA), and conduct dynamic detection.

　　Result: Both adhesion scoring methods showed that typical pelvic adhesions were formed 5 days after modeling. Compared with the blank control group and sham operation, the tPA content of pelvic adhesion peritoneum tissue in each model group was significantly reduced after 5 days. After modeling (P\u003c0.01), the tPA content showed an upward trend 28 days after the model was constructed, but it was still significantly lower than the blank control group and the sham operation group (P\u003c0.01).

　　Conclusion: The EMs pelvic adhesion model is established by transplanting the endometrium into the mesentery through autologous transplantation. The mature model is 5 days after modeling.