动物造模,氟中毒大鼠模型 z-bio 2021-05-27 536

　　Objective: How to establish male and female fluorosis rat animal models and study the gender differences in fluorosis?

　　Method: Select 48 clean SD rats, weighing about 150 g, and divide them into a control group and a fluoride exposure group. The model is free. The fluorine content of the feed that eats fluorine-containing feed is 100 mg/kg. Dynamic observation of the condition, weight and daily changes of fluorosis every three days. When all women develop fluorosis, blood is drawn from the femoral artery. Rats and 110-day male rats. Fluoride ion selective electrode method was used to detect fluoride in urine and fluoride in bone of a batch of rats?

　　Result: The male and female rats in the control group did not show dental fluorosis throughout the experiment. Male and female rats in the fluoride exposure group first developed obvious fluorosis about 60 and 80 days after fluoride exposure. Compared with females, the first appearance of fluorosis in male rats was delayed by about 20 days. mouse. About 30 days after being exposed to fluoride, the male and female rats entered the rapid growth stage, and the weight difference between male and female rats was significant (P\u003c0.01)? In the case of long-term exposure to fluorine, is the ratio of male and female rats in the 110-day fluorine exposure group higher than that in the control group? With the prolongation of fluorine exposure time, the urine fluoride and bone fluoride of male and female rats gradually increased, and the fluorine exposure group increased significantly. Significantly (P\u003c0.01), is there a positive correlation between urine fluoride and bone fluoride and fluoride exposure time? The content of fluoride and bone fluoride in the urine of male rats is higher than that of females, and the difference is statistically significant (P\u003c0.01). Urinary fluorosis and bone fluorosis are affected by fluoride. Gender influence?

　　Conclusion: What are the body weights of male and female rat models suffering from fluorosis? When does fluorosis appear? Are there gender differences in fluoride levels between fluorosis and bones?